Control of lymphokine expression in T helper 2 cells.
نویسندگان
چکیده
In this report we present evidence that Con A-activated T helper 2 cells transcribe the interleukin 2 (IL-2) gene when cycloheximide is added 3 hr after stimulation. This IL-2 mRNA can be translated into functional protein. However, no steady-state mRNA for gamma-interferon can be detected under the same experimental conditions. Furthermore, when cycloheximide is administered at time 0 of activation, no IL-2 mRNA is seen. Nuclear run-on experiments indicate that IL-2 transcription in T helper 2 cells depends on the presence of cycloheximide. It is likely, therefore, that activation of T helper 2 cells leads to the production of a labile regulatory protein that represses the expression of the IL-2 gene. These findings provide insight into the molecular mechanism that governs the differentiation of interleukin 4-producing T helper 2 cells from IL-2-producing precursor cells.
منابع مشابه
Heterogeneity of helper/inducer T lymphocytes. I. Lymphokine production and lymphokine responsiveness
Antigen-specific, Ia-restricted helper/inducer T lymphocytes consist of subsets that can be distinguished by lymphokine secretion. One, called Th1, secretes IL-2 and the other, termed Th2, produces BSF-1/IL-4 in response to stimulation by lectin or antigen receptor signals, and each uses the respective lymphokine as its autocrine growth factor. Cloned lines representing Th2 cells proliferate in...
متن کاملInfluence of 1 Alpha, 25-Dihydroxyvitamin D3 on T Helper 17 Cells and Related Cytokines in Systemic Lupus Erythematosus
Background: Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease. Emerging data suggests that T helper 17 (Th17) cells play a pathogenic role in SLE and the increased number of these cells correlates with disease activity. In recent years, 1α, 25-dihydroxyvitamin D3 (1,25VitD3) has been considered as an immunomodulatory factor. Objective: To investigate the effect of 1,25VitD3...
متن کاملFibroblast and T cells conditioned media induce maturation dendritic cell and promote T helper immune response
Dendritic cells (DCs) induce pathogen-specific T cell responses. We comprehensively studied the effects of addition of maturation stimulus, fibroblasts (fibroblast conditioned medium), PHA activated T cells (T cell conditioned medium), and mixture of fibroblast & PHA activated T cells (FCM-TCCM) conditioned media on maturation of DCs. Monocytes were cultured with GM-CSF and IL-4 for five days. ...
متن کاملEvidence for involvement of dual-function T cells in rejection of MHC class I disparate skin grafts. Assessment of MHC class I alloantigens as in vivo helper determinants
The present study further characterizes the cellular mechanisms involved in the in vivo rejection of MHC class I-disparate skin allografts. Previously, we demonstrated that class I-specific rejection responses could result from collaborations between distinct populations of lymphokine-secreting T helper (Th) and lymphokine-responsive T effector (Teff) cells. In the present study, we have assess...
متن کاملLYMPHOKINE REGULATION OF MACROPHAGE Ia ANTIGEN EXPRESSION Gel Filtration Chromatography
The initiation of many antigen-specific, T-dependent immune responses requires the participation of accessory cells that express Ia antigens (1, 2). Recent evidence indicates that the Ia antigen expression of macrophages, a principle class of accessory cells, is regulated by lymphokines: Ste inman et al. (3) have reported that culture supernatants of Trypanosoma cruzi-activated spleen cells enh...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 86 23 شماره
صفحات -
تاریخ انتشار 1989